Biomarker discovery by stable isotope labeling and quantitative proteomics
نویسندگان
چکیده
منابع مشابه
Stable Isotope Labeling in Proteomics
Proteomics, the analysis of the proteins expressed by a cell, tissue or organism under a specific set of conditions, has undergone a tremendous period of growth in the past few years. Proteomic studies are typically designed to analyze hundreds or thousands of proteins in a single analysis and aim to provide a global view of changes in protein expression that occur in different cellular growth ...
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Quantitative analysis of protein expression is an important tool for the examination of complex biological systems. Albeit its importance, quantitative proteomics is still a challenging task because of the high dynamic range of protein amounts in the cell and the variation in the physical properties of proteins. Stable isotope labeling by amino acids in cell culture (SILAC) has been successfull...
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The detection of doping with recombinant peptide and protein hormones (e.g. human growth hormone -hGH, erythropoietin Epo) is one of the most challenging analytical problems in doping control. Rapid metabolic degradation paired with a low medical dosing strategy reduces the time window for a successful direct detection by immunological methods in the case of hGH to just a few hours after the la...
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Stable isotope labeling is widely used to encode and quantify proteins in mass-spectrometry-based proteomics. We compared metabolic labeling with stable isotope labeling by amino acids in cell culture (SILAC) and chemical labeling by stable isotope dimethyl labeling and find that they have comparable accuracy and quantitative dynamic range in unfractionated proteome analyses and affinity pull-d...
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Spectral counting, a promising method for quantifying relative changes in protein abundance in mass spectrometry-based proteomic analysis, was compared to metabolic stable isotope labeling using (15)N/(14)N "heavy/light" peptide pairs. The data were drawn primarily from a Methanococcus maripaludis experiment comparing a wild-type strain with a mutant deficient in a key enzyme relevant to energy...
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ژورنال
عنوان ژورنال: The FASEB Journal
سال: 2010
ISSN: 0892-6638,1530-6860
DOI: 10.1096/fasebj.24.1_supplement.902.3